Genomic microsatellite characteristics analysis of Dysomma anguillare (Anguilliformes, Dysommidae), based on high-throughput sequencing technology

Microsatellite loci were screened from the genomic data of Dysomma anguillare and their composition distribution analysed by bioinformatics for first time. The results showed that 4,060,742 scaffolds with a total length 1,562 Mb obtained high-throughput sequencing 1,160,104 microsatellite MISA screening, which distributed on 770,294 scaffolds. occurrence frequency relative abundance 28.57% 743/Mb, respectively. Amongst six complete types, dinucleotide repeats accounted largest proportion (592,234, 51.05%), highest (14.58%) (379.27/Mb). A 1488 detected in genome D. , amongst hexanucleotide repeat motifs most abundant (608), followed pentanucleotide (574), tetranucleotide (232), trinucleotide (59), (11) mononucleotide (4). microsatellites same type decreased increase copy numbers. types nucleotide repeats, preponderance repeated are (191,390, 43.77%), CA (150,240, 25.37%), AAT (13,168, 14.05%), CACG (2,649, 8.14%), TAATG (119, 19.16%) CCCTAA (190, 19.16%, 7.65%), number, different this study, would lay foundation development high-quality markers future.